부교수
이현우 Rhee, Hyun-Woo
시스템 화학생물학 연구실
LAB Website
LAB Website
OFFICE
503동 124호
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02-880-4390
LAB
503-103
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02-880-2679
E-MAIL rheehw@snu.ac.kr
연구내용
In a human cell, around 20,000 different human proteins are known to be expressed. These proteins are not uniformly distributed throughout the cell, but they are located in highly specific targeted spaces and form a variety of microenvironments. To map localized protein information in a sub-cellular "space of interest", we develop a new protein mapping method based on compatible protein label chemistry in living cellular physiology. This new method called proximity labeling has the potential to uncover new protein components that are not mapped by traditional protein purification methods such as co-immunoprecipitation. This method can be extended to uncover spatiotemporal subcellular distribution of other biomolecules such as nucleic acids and metabolites in living cells and in live animals.
학력
- Ph.D. Organic Chemistry, Seoul National University, Korea, 2009
- B.S. Biological Sciences and Chemistry (Double Major), Seoul National University, Korea, 2004
주요 경력
- Associate Professor, UNIST Chemistry, Korea, 2017-2018
- Assistant Professor, UNIST Chemistry, Korea, 2013-2017
- Postdoctoral Researcher, MIT Chemistry, USA, 2010-2012
주요 논문
- “Contact-ID, a new tool for profiling organelle contact site, reveals regulatory proteins of mitochondrial-associated membrane formation” Kwak C†, Shin S†, Park JS†, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH, Park SK*, Mun JY*, Kim JS*, and Rhee HW*, PNAS, 2020, 117, 12109-12120
- “Architecture Mapping of the Inner Mitochondrial Membrane Proteome by Cheimcal Tools in Live Cells.” Lee SY, Kang MG, Shin S, Kwak C, Kwon T, Seo JK*, Kim JS*, and Rhee HW*, J. Am. Chem. Soc. 2017, 139, 3651-3662.
- “Structure-Guided Synthesis of a Protein-Based Fluorescent Sensor for Alkyl Halides”, Kang MG†, Lee H†, Kim BH, Dunbayev Y, Seo JK, Lee C*, Rhee HW*, Chem. Commun. 2017, 53, 9226-9229.
- “Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells.” Lee SY†, Lee H†, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK*, Lee C*, and Rhee HW* ACS Cent. Sci. 2016, 2, 506-516.
- “In Cellulo Mapping of Subcellular Localized Bilirubin.” Park JS, Nam E, Lee HK, Lim MH*, and Rhee HW* ACS Chem. Biol. 2016, 11, 2177-2185.
- “APEX fingerprinting reveals the subcellular localization of proteins of interest.” Lee SY, Kang MG, Park JS, Lee G, Ting AY*, Rhee HW* Cell Rep. 2016, 15, 1837-1847
논문 검색
Hyobin Kim, Kwang-eun Kim, Esha Madan, Patrick Martin, Rajan Gogna, Hyun-Woo Rhee, Kyoung-Jae Won
Myeong-Gyun Kang, Hwa-Ryeon Kim, Hee Yong Lee, Chulhwan Kwak, Hyewon Koh, Byoung Heon Kang, Jae-Seok Roe, Hyun-Woo Rhee
Sanghee Shin, Song-Yi Lee, Myeong-Gyun Kang, Dong-Gi Jang, Jeesoo Kim, Hyun-Woo Rhee, Jong-Seo Kim
34. FLEX: genetically encodable enzymatic fluorescence signal amplification using engineered peroxidase
Nirmali Sharma, Minkyo Jung, Pratyush Kumar Mishra, Ji Young Mun, Hyun-Woo Rhee
Yun-Bin Lee, Hyun-Woo Rhee
32. OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools
Ara Lee, Gihyun Sung, Sanghee Shin, Song-Yi Lee, Jaehwan Sim, Truong Thi My Nhung, Tran Diem Nghi, Sang Ki Park, Ponnusamy Pon Sathieshkumar, Imkyeung Kang, Ji Young Mun, Jong-Seo Kim, Hyun-Woo Rhee, Kyeng Min Park, Kimoon Kim
i Eon Kim, So-Young Park, Chulhwan Kwak, Yoonji Lee, Dae-Geun Song, Jae Woo Jung, Haesong Lee, Eun-Ae Shin, Yangie Pinanga, Kyung-hee Pyo, Eun Hae Lee, Wonsik Kim, Soyeon Kim, Chang-Duck Jun, Jeanho Yun, Sun Choi, Hyun-Woo Rhee, Kwang-Hyeon Liu, Jung Weon Lee
June Heo, Yeon-Ji Park, Yonghyeon Kim, Ho-Soo Lee, Jeongah Kim, Soon-Hwan Kwon, Myeong-Gyun Kang, Hyun-Woo Rhee, Woong Sun, Jae-Ho Lee, Hyeseong Cho
Isaac Park, Kwang-eun Kim, Jeesoo Kim, Ae-Kyeong Kim, Subin Bae, Minkyo Jung, Jinhyuk Choi, Pratyush Kumar Mishra, Taek-Min Kim, Chulhwan Kwak, Myeong-Gyun Kang, Chang-Mo Yoo, Ji Young Mun, Kwang-Hyeon Liu, Kyu-Sun Lee, Jong-Seo Kim, Jae Myoung Suh, Hyun-Woo Rhee
Nari Kim, Tae-Hyeon Kim, Chaelim Kim, Jee-Eun Lee, Myeong-Gyun Kang, Sanghee Shin, Minkyo Jung, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee, Seung-Yeol Park, Yongdae Shin, Joo-Yeon Yoo