Development of Antibody-like Protein Capture Agents
소속 :
연사 : 임재홍 박사 (Institute of Bioengineering and Nanotechnology)
일시 : 2009-05-15 11:00 ~
장소 : 200동 2004호
일시: 2009년 5월 15일 오전 11:00
장소: 200동 2004호
-Abstract-
It is of great interest to equip high-affinity protein capture agents for many purposes. Despite recent studies on oligonucleotide aptamers and phage-display peptides, only antibody has been the realistic tool for reliable binding to proteins of physiological significance to date. However, its availability is greatly limited due to high cost, low stability, and complicated production. We develop a further alternative that is the use of one-bead.one-compound (OBOC) peptide or peptidomimetic libraries. Overcoming insufficient affinity and specificity from typical OBOC libraries containing limited peptide length and chemical diversity, we utilize in situ click chemistry as a screening approach towards the construction of multiligand protein-capture agents. We harnessed the method to fish out a biligand capture agent against bovine carbonic anhydrase II (bCAII) as a model system. The entire process has been streamlined for library synthesis, assay, sorting, and sequencing to suit for a high throughput development. This application can be used as affinity reagents and molecular probes for in vitro diagnosis and drug discovery.
장소: 200동 2004호
-Abstract-
It is of great interest to equip high-affinity protein capture agents for many purposes. Despite recent studies on oligonucleotide aptamers and phage-display peptides, only antibody has been the realistic tool for reliable binding to proteins of physiological significance to date. However, its availability is greatly limited due to high cost, low stability, and complicated production. We develop a further alternative that is the use of one-bead.one-compound (OBOC) peptide or peptidomimetic libraries. Overcoming insufficient affinity and specificity from typical OBOC libraries containing limited peptide length and chemical diversity, we utilize in situ click chemistry as a screening approach towards the construction of multiligand protein-capture agents. We harnessed the method to fish out a biligand capture agent against bovine carbonic anhydrase II (bCAII) as a model system. The entire process has been streamlined for library synthesis, assay, sorting, and sequencing to suit for a high throughput development. This application can be used as affinity reagents and molecular probes for in vitro diagnosis and drug discovery.
