Faculty

DEPARTMENT OF CHEMISTRY, SEOUL NATIONAL UNIVERSITY.

Associate Professor

Lee, Nam Ki

Single-Molecule & Cell Dynamics
LAB Website
OFFICE 503동 420호 / +82-2-880-2234
LAB 503-403,132 / +82-2-880-2235

Research Topic

The main research interest of our group is developing new single-molecule and single-cell imaging techniques for investigating various biological phenomena. We are using single-molecule FRET, single-vesicle assay, single-protein detection in a living cell, real-time single-protein tracking, and super-resolution imaging. Using these techniques, we are directly visualizing the cellular processes, such as transcriptional dynamics and the movement of DNA, in a living cell. We are also heavily investigating membrane protein interactions and their diffusional mode in a living cell. Not only the living cell system, we are also studying protein-DNA interactions at the single-molecule level. Finally, we are studying the molecular mechanism of Parkinson’s disease based on the interactions between alpha-Synuclein and SNARE-proteins

Education

  • Ph.D. Chemistry, Seoul National University, Seoul, Korea, 2005
  • M.S. Chemistry, Seoul National University, Seoul, Korea, 2000
  • B.S. Chemistry, Seoul National University, Seoul, Korea, 1998

Career

  • Associate Professor, Department of Chemistry, Seoul National University, Seoul, Korea, 2017-Present
  • Associate Professor, Department of Physics & School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea, 2013-2017
  • Assistant Professor, Department of Physics & School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea, 2009-2013
  • Postdoctoral Fellow, Harvard University, Cambridge, USA, 2006-2008
  • Visiting Scientist, U. C. Los Angeles, California, USA, 2002-2003

Papers

  1. "Single-molecule observation of ATP-independent SSB displacement by RecO in Deinococcus radiodurans", J. Hwang, J.-Y. Kim, C. Kim, S. park, S. Joo, S. K. Kim*, N. K. Lee*, eLife, 2020, 9, e50945.
  2. "Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E.coli.” S. Yang, S. H. Kim, D.-K. Kim, H. J. An, J. B. Son, A. H. Gynnå, N. K. Lee, Nat. Commun. 2019, 10, 5131.
  3. “Dynamic release of bending stress in short dsDNA by a kink and forks”, C. Kim, O. Lee, J.-Y. Kim, W. Sung, N. K. Lee, Angew. Chem. Int. Ed. 2015, 31, 8943-8947.
  4. “Real-time submillisecond single-molecule FRET dynamics of freely diffusing molecules with liposome tethering”, J.-Y. Kim, C. Kim, N. K. Lee, Nat. Commun. 2015, 6, 6992.
  5. “Contribution of RNA polymerase concentration variation to protein expression noise”, S. Yang, S. H. Kim, Y. R. Lim, C. H. Kim, H. J. An, J-H Kim, J. Sung,* N. K Lee*, Nat. Commun. 2014, 5, 4761.
  6. “Large α-Synuclein oligomers inhibit neuronal SNARE-mediated vesicle docking”, B. K Choi, M. G. Choi, J. Y. Kim, Y. Yang, Y. Lai, D. H. Kweon, N. K. Lee,* Y. K. Shin*, Proc. Natl. Acad. Sci. USA 2013, 110, 4087-4092.

Search Papers

Jaehun Yi, Sanghun Yeou, Nam Ki Lee
Journal of the American Chemical Society, 2022, 144, 29, (2022-07-27) Link
Sanghun Yeou, Jihee Hwang, Jaehun Yi, Cheolhee Kim, Seong Keun Kim, Nam Ki Lee
Chemical Science, 2022, 13, (2022-06-02) Link
Sanghun Yeou, Nam Ki Lee
Molecules and Cells, 2022, 45, 1, (2022-03-31) Link
Dong-Kyun Kim, Young Sook Kim, Chan Sik Kim, Nam Ki Lee
Bulletin of the Korean Chemical Society, 2021, 42, 3, (20210301) Link
Yoonjung Cho, Hyeong Jeon An, Taehoon Kim, Chulbom Lee, Nam Ki Lee
Journal of the American Chemical Society, 2021, 143, 35, (2021-09-08) Link
Sanghun Yeou, Nam Ki Lee
Bulletin of the Korean Chemical Society, 2021, 42, 8, (2021-08) Link
Ryan Khounlo, Brenden J. D. Hawk, Tung-Mei Khu, Gyeongji Yoo, Nam Ki Lee, Josh Pierson, Yeon-Kyun Shin
Frontiers in Cell and Developmental Biology, 2021, 9, (2021-07-19) Link
Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N Kapanidis, Harold D Kim, Ted Laurence, Nam Ki Lee, Tae-Hee Lee, Edward A Lemke, Emmanuel Margeat, Jens Michaelis, Xavier Michalet, Sua Myong, Daniel Nettels, Thomas-Otavio Peulen, Evelyn Ploetz, Yair Razvag, Nicole C Robb, Benjamin Schuler, Hamid Soleimaninejad, Chun Tang, Reza Vafabakhsh, Don C Lamb, Claus Am Seidel, Shimon Weiss
eLife, 2021, 10, e60416, (2021-05-29) Link
Gyeongji Yoo, Sanghun Yeou, Jung Bae Son, Yeon-Kyun Shin, Nam Ki Lee
Scientific Reports, 2021, 11, 10955, (2021-05-26) Link
Jihee Hwang, Jae-Yeol Kim, Cheolhee Kim, Soojin Park, Sungmin Joo, Seong Keun Kim, Nam Ki Lee
eLife, 2020, 9, (2020-04-16) Link