Faculty

DEPARTMENT OF CHEMISTRY, SEOUL NATIONAL UNIVERSITY.

Professor

Lee, Nam Ki

Single-Molecule & Cell Dynamics
LAB Website
OFFICE 503동 423호 / +82-2-880-2234
LAB 503-403,132 / +82-2-880-2235

Research Topic

The main research interest of our group is developing new single-molecule and single-cell imaging techniques for investigating various biological phenomena. We are using single-molecule FRET, single-vesicle assay, single-protein detection in a living cell, real-time single-protein tracking, and super-resolution imaging. Using these techniques, we are directly visualizing the cellular processes, such as transcriptional dynamics and the movement of DNA, in a living cell. We are also heavily investigating membrane protein interactions and their diffusional mode in a living cell. Not only the living cell system, we are also studying protein-DNA interactions at the single-molecule level. Finally, we are studying the molecular mechanism of Parkinson’s disease based on the interactions between alpha-Synuclein and SNARE-proteins

Education

  • Ph.D. Chemistry, Seoul National University, Seoul, Korea, 2005
  • M.S. Chemistry, Seoul National University, Seoul, Korea, 2000
  • B.S. Chemistry, Seoul National University, Seoul, Korea, 1998

Career

  • Associate Professor, Department of Chemistry, Seoul National University, Seoul, Korea, 2017-Present
  • Associate Professor, Department of Physics & School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea, 2013-2017
  • Assistant Professor, Department of Physics & School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea, 2009-2013
  • Postdoctoral Fellow, Harvard University, Cambridge, USA, 2006-2008
  • Visiting Scientist, U. C. Los Angeles, California, USA, 2002-2003

Papers

  1. "Single-molecule observation of ATP-independent SSB displacement by RecO in Deinococcus radiodurans", J. Hwang, J.-Y. Kim, C. Kim, S. park, S. Joo, S. K. Kim*, N. K. Lee*, eLife, 2020, 9, e50945.
  2. "Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E.coli.” S. Yang, S. H. Kim, D.-K. Kim, H. J. An, J. B. Son, A. H. Gynnå, N. K. Lee, Nat. Commun. 2019, 10, 5131.
  3. “Dynamic release of bending stress in short dsDNA by a kink and forks”, C. Kim, O. Lee, J.-Y. Kim, W. Sung, N. K. Lee, Angew. Chem. Int. Ed. 2015, 31, 8943-8947.
  4. “Real-time submillisecond single-molecule FRET dynamics of freely diffusing molecules with liposome tethering”, J.-Y. Kim, C. Kim, N. K. Lee, Nat. Commun. 2015, 6, 6992.
  5. “Contribution of RNA polymerase concentration variation to protein expression noise”, S. Yang, S. H. Kim, Y. R. Lim, C. H. Kim, H. J. An, J-H Kim, J. Sung,* N. K Lee*, Nat. Commun. 2014, 5, 4761.
  6. “Large α-Synuclein oligomers inhibit neuronal SNARE-mediated vesicle docking”, B. K Choi, M. G. Choi, J. Y. Kim, Y. Yang, Y. Lai, D. H. Kweon, N. K. Lee,* Y. K. Shin*, Proc. Natl. Acad. Sci. USA 2013, 110, 4087-4092.

Search Papers

Eitan Lerner, Anders Barth, Jelle Hendrix, Benjamin Ambrose, Victoria Birkedal, Scott C Blanchard, Richard Börner, Hoi Sung Chung, Thorben Cordes, Timothy D Craggs, Ashok A Deniz, Jiajie Diao, Jingyi Fei, Ruben L Gonzalez, Irina V Gopich, Taekjip Ha, Christian A Hanke, Gilad Haran, Nikos S Hatzakis, Sungchul Hohng, Seok-Cheol Hong, Thorsten Hugel, Antonino Ingargiola, Chirlmin Joo, Achillefs N Kapanidis, Harold D Kim, Ted Laurence, Nam Ki Lee, Tae-Hee Lee, Edward A Lemke, Emmanuel Margeat, Jens Michaelis, Xavier Michalet, Sua Myong, Daniel Nettels, Thomas-Otavio Peulen, Evelyn Ploetz, Yair Razvag, Nicole C Robb, Benjamin Schuler, Hamid Soleimaninejad, Chun Tang, Reza Vafabakhsh, Don C Lamb, Claus Am Seidel, Shimon Weiss
eLife, 2021, 10, e60416, (2021-05-29) Link
Gyeongji Yoo, Sanghun Yeou, Jung Bae Son, Yeon-Kyun Shin, Nam Ki Lee
Scientific Reports, 2021, 11, 10955, (2021-05-26) Link
Jihee Hwang, Jae-Yeol Kim, Cheolhee Kim, Soojin Park, Sungmin Joo, Seong Keun Kim, Nam Ki Lee
eLife, 2020, 9, (2020-04-16) Link
Sora Yang, Seunghyeon Kim, Dong-Kyun Kim, Hyeong Jeon An, Jung Bae Son, Nam Ki Lee
Nature Communications, 2019, 10, 1, (2019-11-12) Link
Do-Hyeon Kim, Soyeon Park, Dong-Kyun Kim, Min Gyu Jeong, Jungeun Noh, Yonghoon Kwon, Kai Zhou, Nam Ki Lee, Sung Ho Ryu
PLOS BIOLOGY, 2018, 16, 12, (2018-12-13) Link
Björn Hellenkamp, Sonja Schmid , Olga Doroshenko, Oleg Opanasyuk, Ralf Kühnemuth, Nam Ki Lee, 외53명, Jens Michaelis, Claus A. M. Seidel , Timothy D. Craggs, Thorsten Hugel
Nature Methods, 2018, 15, 9, (2018-09-01) Link
Keewon Sung, Jinho Park, Younggyu Kim, Nam Ki Lee, Seong Keun Kim
Journal of The American Chemical Society, 2018, 140, 25, (2018-06-27) Link
Mi-Jeong Kwak, J. Dongun Kim, Hyunmin Kim, Cheolhee Kim, James W. Bowman, Seonghoon Kim, Keehyoung Joo, Jooyoung Lee, Kyeong Sik Jin, Yeon-Gil Kim, Nam Ki Lee, Jae U. Jung, Byung-Ha Oh
Nature Microbiology, 2017, 2, (2017-07-17) Link
Do-Hyeon Kim, Dong-Kyun Kim, Kai Zhou, Soyeon Park, Yonghoon Kwon, Min Gyu Jeong, Nam Ki Lee, Sung Ho Ryu
Chemical Science, 2017, 8, 7, (2017-07-01) Link
Sungwook Jung, Joonhyuck Park, Jiwon Bang, Jae-Yeol Kim, Cheolhee Kim, Yongmoon Jeon, Seung Hwan Lee, Ho Jin, Sukyung Choi, Bomi Kim, Woo Jin Lee, Chan-Gi Pack, Jong-Bong Lee, Nam Ki Lee, Sungjee Kim
Journal of The American Chemical Society, 2017, 139, 22, (2017-06-07) Link