Associate Professor

Rhee, Hyun-Woo

Systems Chemical Biology Laboratory
LAB Website
OFFICE 503동 124호 / +82-2-880-4390
LAB 503-103 / +82-2-880-2679

Research Topic

In a human cell, around 20,000 different human proteins are known to be expressed. These proteins are not uniformly distributed throughout the cell, but they are located in highly specific targeted spaces and form a variety of microenvironments. To map localized protein information in a sub-cellular "space of interest", we develop a new protein mapping method based on compatible protein label chemistry in living cellular physiology. This new method called proximity labeling has the potential to uncover new protein components that are not mapped by traditional protein purification methods such as co-immunoprecipitation. This method can be extended to uncover spatiotemporal subcellular distribution of other biomolecules such as nucleic acids and metabolites in living cells and in live animals.


  • Ph.D. Organic Chemistry, Seoul National University, Korea, 2009
  • B.S. Biological Sciences and Chemistry (Double Major), Seoul National University, Korea, 2004


  • Associate Professor, UNIST Chemistry, Korea, 2017-2018
  • Assistant Professor, UNIST Chemistry, Korea, 2013-2017
  • Postdoctoral Researcher, MIT Chemistry, USA, 2010-2012


  1. “Contact-ID, a new tool for profiling organelle contact site, reveals regulatory proteins of mitochondrial-associated membrane formation” Kwak C†, Shin S†, Park JS†, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH, Park SK*, Mun JY*, Kim JS*, and Rhee HW*, PNAS, 2020, 117, 12109-12120
  2. “Architecture Mapping of the Inner Mitochondrial Membrane Proteome by Cheimcal Tools in Live Cells.” Lee SY, Kang MG, Shin S, Kwak C, Kwon T, Seo JK*, Kim JS*, and Rhee HW*, J. Am. Chem. Soc. 2017, 139, 3651-3662.
  3. “Structure-Guided Synthesis of a Protein-Based Fluorescent Sensor for Alkyl Halides”, Kang MG†, Lee H†, Kim BH, Dunbayev Y, Seo JK, Lee C*, Rhee HW*, Chem. Commun. 2017, 53, 9226-9229.
  4. “Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells.” Lee SY†, Lee H†, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK*, Lee C*, and Rhee HW* ACS Cent. Sci. 2016, 2, 506-516.
  5. “In Cellulo Mapping of Subcellular Localized Bilirubin.” Park JS, Nam E, Lee HK, Lim MH*, and Rhee HW* ACS Chem. Biol. 2016, 11, 2177-2185.
  6. “APEX fingerprinting reveals the subcellular localization of proteins of interest.” Lee SY, Kang MG, Park JS, Lee G, Ting AY*, Rhee HW* Cell Rep. 2016, 15, 1837-1847

Search Papers

Yun-Bin Lee, Hyun-Woo Rhee
Trends in Biochemical Sciences, 2024, 49, 3, (2024-03-07) Link
Ara Lee, Gihyun Sung, Sanghee Shin, Song-Yi Lee, Jaehwan Sim, Truong Thi My Nhung, Tran Diem Nghi, Sang Ki Park, Ponnusamy Pon Sathieshkumar, Imkyeung Kang, Ji Young Mun, Jong-Seo Kim, Hyun-Woo Rhee, Kyeng Min Park, Kimoon Kim
Nature Communications, 2024, 15, 1, (2024-02-29) Link
i Eon Kim, So-Young Park, Chulhwan Kwak, Yoonji Lee, Dae-Geun Song, Jae Woo Jung, Haesong Lee, Eun-Ae Shin, Yangie Pinanga, Kyung-hee Pyo, Eun Hae Lee, Wonsik Kim, Soyeon Kim, Chang-Duck Jun, Jeanho Yun, Sun Choi, Hyun-Woo Rhee, Kwang-Hyeon Liu, Jung Weon Lee
Cancer Communication, 2023, (2023-12-22) Link
June Heo, Yeon-Ji Park, Yonghyeon Kim, Ho-Soo Lee, Jeongah Kim, Soon-Hwan Kwon, Myeong-Gyun Kang, Hyun-Woo Rhee, Woong Sun, Jae-Ho Lee, Hyeseong Cho
Cell Death & Disease, 2023, 14, 788, (2023-12-01) Link
Isaac Park, Kwang-eun Kim, Jeesoo Kim, Ae-Kyeong Kim, Subin Bae, Minkyo Jung, Jinhyuk Choi, Pratyush Kumar Mishra, Taek-Min Kim, Chulhwan Kwak, Myeong-Gyun Kang, Chang-Mo Yoo, Ji Young Mun, Kwang-Hyeon Liu, Kyu-Sun Lee, Jong-Seo Kim, Jae Myoung Suh, Hyun-Woo Rhee
Nature Chemical Biology, 2023, (2023-10-26) Link
Nari Kim, Tae-Hyeon Kim, Chaelim Kim, Jee-Eun Lee, Myeong-Gyun Kang, Sanghee Shin, Minkyo Jung, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee, Seung-Yeol Park, Yongdae Shin, Joo-Yeon Yoo
Developmental Cell, 2023, 58, 19, (2023-10-09) Link
Pratyush Kumar Mishra, Nirmali Sharma, Hyunwoo Kim, Changwook Lee, Hyun-Woo Rhee
ACS Central Science, 2023, 9, 8, (2023-08-23) Link
Anna Park, Kwang-eun Kim, Isaac Park, Sang Heon Lee, Kun-Young Park, Minkyo Jung, Xiaoxu Li, Maroun Bou Sleiman, Su Jeong Lee, Dae-Soo Kim, Jaehoon Kim, Dae-Sik Lim, Eui-Jeon Woo, Eun Woo Lee, Baek Soo Han, Kyoung-Jin Oh, Sang Chul Lee, Johan Auwerx, Ji Young Mun, Hyun-Woo Rhee, Won Kon Kim, Kwang-Hee Bae, Jae Myoung Suh
Nature Communications, 2023, 14, (2023-06-23) Link
Yejin Kim, Chulhwan Kwak, Jong Bae Park, Do-Hyun Nam, Hyun-Woo Rhee, Sung Soo Kim
Advanced Therapeutics, 2023, (2023-05-23) Link
Sun-Il Yun, Chulhwan Kwak, Song-Yi Lee, Sanghee Shin, Changsuk Oh, Jong-Seo Kim, Hyun-Woo Rhee, Kyeong Kyu Kim
FASEB Journal, 2023, 37, 5, (2023-05-01) Link