Associate Professor

Rhee, Hyun-Woo

Systems Chemical Biology Laboratory
LAB Website
OFFICE 503동 124호 / +82-2-880-4390
LAB 503-103 / +82-2-880-2679

Research Topic

In a human cell, around 20,000 different human proteins are known to be expressed. These proteins are not uniformly distributed throughout the cell, but they are located in highly specific targeted spaces and form a variety of microenvironments. To map localized protein information in a sub-cellular "space of interest", we develop a new protein mapping method based on compatible protein label chemistry in living cellular physiology. This new method called proximity labeling has the potential to uncover new protein components that are not mapped by traditional protein purification methods such as co-immunoprecipitation. This method can be extended to uncover spatiotemporal subcellular distribution of other biomolecules such as nucleic acids and metabolites in living cells and in live animals.


  • Ph.D. Organic Chemistry, Seoul National University, Korea, 2009
  • B.S. Biological Sciences and Chemistry (Double Major), Seoul National University, Korea, 2004


  • Associate Professor, UNIST Chemistry, Korea, 2017-2018
  • Assistant Professor, UNIST Chemistry, Korea, 2013-2017
  • Postdoctoral Researcher, MIT Chemistry, USA, 2010-2012


  1. “Contact-ID, a new tool for profiling organelle contact site, reveals regulatory proteins of mitochondrial-associated membrane formation” Kwak C†, Shin S†, Park JS†, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH, Park SK*, Mun JY*, Kim JS*, and Rhee HW*, PNAS, 2020, 117, 12109-12120
  2. “Architecture Mapping of the Inner Mitochondrial Membrane Proteome by Cheimcal Tools in Live Cells.” Lee SY, Kang MG, Shin S, Kwak C, Kwon T, Seo JK*, Kim JS*, and Rhee HW*, J. Am. Chem. Soc. 2017, 139, 3651-3662.
  3. “Structure-Guided Synthesis of a Protein-Based Fluorescent Sensor for Alkyl Halides”, Kang MG†, Lee H†, Kim BH, Dunbayev Y, Seo JK, Lee C*, Rhee HW*, Chem. Commun. 2017, 53, 9226-9229.
  4. “Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells.” Lee SY†, Lee H†, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK*, Lee C*, and Rhee HW* ACS Cent. Sci. 2016, 2, 506-516.
  5. “In Cellulo Mapping of Subcellular Localized Bilirubin.” Park JS, Nam E, Lee HK, Lim MH*, and Rhee HW* ACS Chem. Biol. 2016, 11, 2177-2185.
  6. “APEX fingerprinting reveals the subcellular localization of proteins of interest.” Lee SY, Kang MG, Park JS, Lee G, Ting AY*, Rhee HW* Cell Rep. 2016, 15, 1837-1847

Search Papers

Jiyoon Beon, Sungwook Han, Hyeokjun Yang, Seung Eun Park, Kwangbeom Hyun, Song-Yi Lee, Hyun-Woo Rhee, Jeong Kon Seo, Jaehoon Kim, Seyun Kim, Daeyoup Lee
eLife, 2022, 11, (2022-06-16) Link
Pratyush Kumar Mishra, Myeong-Gyun Kang, Hakbong Lee, Seungjoon Kim, Subin Choi, Nirmali Sharma, Cheol-Min Park, Jaewon Ko, Changwook Lee, Jeong Kon Seo, Hyun-Woo Rhee
Chemical Science, 2022, 13, 4, (2022-01-28) Link
Jee Hyung Sohn, Yul Ji, Chang-Yun Cho, Hahn Nahmgoong, Sangsoo Lim, Yong Geun Jeon, Sang Mun Han, Ji Seul Han, Isaac Park, Hyun-Woo Rhee, Sun Kim, Jae Bum Kim
Diabetes, 2022, 70, 12, (2021-12-01) Link
Chang-Ryul Choi, Hyun-Woo Rhee
Trends in Biotechnology, 2021, (2021-10-16) Link
Kwang-eun Kim, Isaac Park, Jeesoo Kim, Myeong-Gyun Kang, Won Gun Choi, Hyemi Shin, Jong-Seo Kim, Hyun-Woo Rhee, Jae Myoung Suh
Nature Communications, 2021, 5204, (2021-09-01) Link
Yu Geon Lee, Hui Won Kim, Yeji Nam, Kyeong Jin Shin, Yu Jin Lee, Do Hong Park, Hyun-Woo Rhee, Jeong Kon Seo, Young Chan Chae
Oncogenesis, 2021, 10, 18, (2021-02-26) Link
Chaiheon Lee, Jung Seung Nam, Chae Gyu Lee, Mingyu Park, Chang-Mo Yoo, Hyun-Woo Rhee, Jeong Kon Seo, Tae-Hyuk Kwon
Nature Communications , 2021, 12, 26, (2021-01-04) Link
Chulhwan Kwak, Sanghee Shin, Jong-Seok Park, Minkyo Jung, Truong Thi My Nhung, Myeong-Gyun Kang, Chaiheon Lee, Tae-Hyuk Kwon, Sang Ki Park, Ji Young Mune, Jong-Seo Kim, Hyun-Woo Rhee
Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, 22, (2020-06-02) Link
Kelvin F. Cho, Tess C. Branon, Sanjana Rajeev, Tanaya Svinkina, Namrata D.Udeshi, Themis Thoudam, Chulhwan Kwak, Hyun-Woo Rhee, In-Kyu Lee, Steven A.Carr, Alice Y. Ting
Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, 22, (2020-06-02) Link
Pratyush Kumar Mishra, Chang‐Mo Yoo, Eunmi Hong, Hyun-Woo Rhee
ChemBioChem, 2020, 21, 7, (2020-04-01) Link