Professor
Rhee, Hyun-Woo
Systems Chemical Biology Laboratory
LAB Website
LAB Website
OFFICE
503동 124호
/
+82-2-880-4390
LAB
503-103
/
+82-2-880-2679
E-MAIL rheehw@snu.ac.kr
Research Topic
In a human cell, around 20,000 different human proteins are known to be expressed. These proteins are not uniformly distributed throughout the cell, but they are located in highly specific targeted spaces and form a variety of microenvironments. To map localized protein information in a sub-cellular "space of interest", we develop a new protein mapping method based on compatible protein label chemistry in living cellular physiology. This new method called proximity labeling has the potential to uncover new protein components that are not mapped by traditional protein purification methods such as co-immunoprecipitation. This method can be extended to uncover spatiotemporal subcellular distribution of other biomolecules such as nucleic acids and metabolites in living cells and in live animals.
Education
- Ph.D. Organic Chemistry, Seoul National University, Korea, 2009
- B.S. Biological Sciences and Chemistry (Double Major), Seoul National University, Korea, 2004
Career
- Professor, SNU Chemistry, Korea, 2025-current
- Associate Professor, SNU Chemistry, Korea, 2018-2025
- Assistant/Associate Professor, UNIST Chemistry, Korea, 2013-2018
- Postdoctoral Researcher, MIT Chemistry, USA, 2010-2012
Papers
- "Spray-Type Modifications: An Emerging Paradigm in Post-translational Modifications" Lee YB, Rhee HW* Trends Biochem. Sci. 2024, 49, 208-223
- "Mitochondrial matrix RTN4IP1/OPA10 is an oxidoreductase for coenzyme Q synthesis" Park I†, Kim KE†, Kim J†, AK Kim, Bae S, Jung M, Choi J, Mishra PK, TM Kim, Kwak C, Kang MG, Yoo CM, Mun JY, Liu KH, Lee KS*, Kim JS*, Suh JM*, Rhee HW* Nat. Chem. Biol. 2024, 20, 221-233
- "Mitochondrial thermogenesis can trigger heat-shock response in the nucleus" Kang MG† Kim HR†, Lee HY†, Kwak C, Koh H, Kang BH, Roe JS*, Rhee HW*, ACS Cent. Sci. 2024, 10, 1231-1241
- "GEN-Click: Genetically Encodable Click Reactions for Spatially Restricted Metabolite Labeling" Mishra PK*, Sharma N, Kim H, Lee C, Rhee HW* ACS Cent. Sci. 2023, 9, 1650–1657
- "Identification of compound-binding target proteins in live cells by proximity labeling (PROCID)," Kwak C†, Park C†, Ko M, Im CY, Moon H, Park YH, Kim SY, Lee S, Kang MG, Kwon HJ*, Hong E*, Seo JK*, Rhee HW* Cell Chem. Biol. 2022, 29, 1739-1753
- “Contact-ID, a new tool for profiling organelle contact site, reveals regulatory proteins of mitochondrial-associated membrane formation” Kwak C†, Shin S†, Park JS†, Jung M, Nhung TTM, Kang MG, Lee C, Kwon TH, Park SK*, Mun JY*, Kim JS*, Rhee HW*, PNAS, 2020, 117, 12109-12120
Search Papers
Nari Kim, Tae-Hyeon Kim, Chaelim Kim, Jee-Eun Lee, Myeong-Gyun Kang, Sanghee Shin, Minkyo Jung, Jong-Seo Kim, Ji Young Mun, Hyun-Woo Rhee, Seung-Yeol Park, Yongdae Shin, Joo-Yeon Yoo
Pratyush Kumar Mishra, Nirmali Sharma, Hyunwoo Kim, Changwook Lee, Hyun-Woo Rhee
Anna Park, Kwang-eun Kim, Isaac Park, Sang Heon Lee, Kun-Young Park, Minkyo Jung, Xiaoxu Li, Maroun Bou Sleiman, Su Jeong Lee, Dae-Soo Kim, Jaehoon Kim, Dae-Sik Lim, Eui-Jeon Woo, Eun Woo Lee, Baek Soo Han, Kyoung-Jin Oh, Sang Chul Lee, Johan Auwerx, Ji Young Mun, Hyun-Woo Rhee, Won Kon Kim, Kwang-Hee Bae, Jae Myoung Suh
Yejin Kim, Chulhwan Kwak, Jong Bae Park, Do-Hyun Nam, Hyun-Woo Rhee, Sung Soo Kim
Sun-Il Yun, Chulhwan Kwak, Song-Yi Lee, Sanghee Shin, Changsuk Oh, Jong-Seo Kim, Hyun-Woo Rhee, Kyeong Kyu Kim
23. A multifunctional peroxidase-based reaction for imaging, sensing and networking of spatial biology
Nirmali Sharma, Pratyush Kumar Mishra, Chang-Mo Yoo, Minkyo Jung, Ji Young Mun, Hyun Woo Rhee
Chulhwan Kwak, Cheolhun Park, Minjeong Ko, Chun Young Im, Heegyum Moon, Young-Hoon Park, So Young Kim, Seungyeon Lee, Myeong-Gyun Kang, Ho Jeong Kwon, Eunmi Hong, Jeong Kon Seo, Hyun-Woo Rhee
Mingle Li, Yunjie Xu, Hyun-Woo Rhee, Jong Seung Kim
Pratyush Kumar Mishra, Issac Park, Nirmali Sharma, Chang-Mo Yoo, Hee Yong Lee, Hyun-Woo Rhee
Jiyoon Beon, Sungwook Han, Hyeokjun Yang, Seung Eun Park, Kwangbeom Hyun, Song-Yi Lee, Hyun-Woo Rhee, Jeong Kon Seo, Jaehoon Kim, Seyun Kim, Daeyoup Lee