Complex metabolic reactions in cells are distributed in organelle modular compartments.
Reactions in organelles have been reproduced in vitro by reconstituting functional protein
machinery into membrane systems. However, it is difficult to maintain and control such a
reaction. We tested harvest organelle design, if switchable, to provide both a sustainable energy
source and means to induce bladder response.[1] ATP (ATP) sinter agent and two light
converters (plant-derived photochemical system II and proteorhodopsin derived from bacteria)
enable ATP synthesis. It depends on two optical conversion devices, and it enables optical
control to dynamically control ATP synthesis. Red light and green light interfere with ATP
synthesis. We encapsulated giant vesicle light synthesizing organelle to form one cell line and
showed two ATP dependent responses, optical control of carbon fixation and actin
polymerization, changing the morphology of the vesicle. We hope to develop a full biomimetic
vesicular system with a regulatory network showing switchable photosynthetic organelle
homeostasis and complex cell behavior.