APEX, a genetically encoded tag for spatial proteome mapping & EM imaging
소속 :
연사 : 이현우 교수 ( UNIST )
일시 : 2013-05-02 16:30 ~
장소 : 500동 목암홀
일 시 : 2013년 5월 2일, 4:30 PM
장 소 : 500동 목암홀
-Abstract-
Microscopy and mass spectrometry (MS) are complementary techniques: the former provides spatiotemporal information in living cells, but only for a handful of recombinant proteins, while the latter can detect thousands of endogenous proteins simultaneously, but only in lysed samples. In this presentation, I will introduce a new technology that combines these strengths by offering spatially- and temporally-resolved proteomic maps of endogenous proteins within living cells. The method relies on a genetically-targetable peroxidase enzyme (APEX) that biotinylates nearby proteins promiscuously, which are subsequently purified and identified by MS. In this seminar, I will present our recent proteomics mapping result of mitochondrial matrix using APEX-generated phenoxyl radical biotinylation in living cells. The radical generation activity of APEX can be very useful to make novel EM contrast as well.
장 소 : 500동 목암홀
-Abstract-
Microscopy and mass spectrometry (MS) are complementary techniques: the former provides spatiotemporal information in living cells, but only for a handful of recombinant proteins, while the latter can detect thousands of endogenous proteins simultaneously, but only in lysed samples. In this presentation, I will introduce a new technology that combines these strengths by offering spatially- and temporally-resolved proteomic maps of endogenous proteins within living cells. The method relies on a genetically-targetable peroxidase enzyme (APEX) that biotinylates nearby proteins promiscuously, which are subsequently purified and identified by MS. In this seminar, I will present our recent proteomics mapping result of mitochondrial matrix using APEX-generated phenoxyl radical biotinylation in living cells. The radical generation activity of APEX can be very useful to make novel EM contrast as well.
