Mechanism of spermatogonial stem cell reprogramming into mGS
소속 :
연사 : 김성태 교수 (고려대)
일시 : 2009-10-01 17:00 ~
장소 : 500동 목암홀
일시: 2009년 10월 1일 오후 5:00
장소: 500동 목암홀
- Abstract -
Germline stem cells are unipotent stem cells ultimately generating sperms through several differentiation steps in the testis. In 2003, Dr. Shinohara group for the first time announced successful mouse spermatogonial stem cell in vitro culture for a long time, which has facilitated the studies using the stem cells as an adult stem cell model. In 2004, the same group reported that pluripotent stem cells spontaneously arose with very low efficiency from germline stem cells in culture in the absence of any genetic modification. The pluripotent stem cells, designated multipotent germline stem cells (mGSs), have since been successfully isolated from various murine strains although underlying mechanism is still unknown. Several research groups have been working on elucidating how this reprogramming is controlled and found out that there may be germline specific mechanism inhibiting expression of major genes associated with pluripotency. Purposesof this study are to uncover mechanism suppressing latent pluripotency and to efficiently control the expression of pluripotent marker genes in germline stem cells without any gene modification for possible use of male germline cell as a substituent for embryonic stem cell. Here I would like to introduce preliminary results that show possible involvement of noncoding RNAs and strategies using gene regulation and chemical libraries.
장소: 500동 목암홀
- Abstract -
Germline stem cells are unipotent stem cells ultimately generating sperms through several differentiation steps in the testis. In 2003, Dr. Shinohara group for the first time announced successful mouse spermatogonial stem cell in vitro culture for a long time, which has facilitated the studies using the stem cells as an adult stem cell model. In 2004, the same group reported that pluripotent stem cells spontaneously arose with very low efficiency from germline stem cells in culture in the absence of any genetic modification. The pluripotent stem cells, designated multipotent germline stem cells (mGSs), have since been successfully isolated from various murine strains although underlying mechanism is still unknown. Several research groups have been working on elucidating how this reprogramming is controlled and found out that there may be germline specific mechanism inhibiting expression of major genes associated with pluripotency. Purposesof this study are to uncover mechanism suppressing latent pluripotency and to efficiently control the expression of pluripotent marker genes in germline stem cells without any gene modification for possible use of male germline cell as a substituent for embryonic stem cell. Here I would like to introduce preliminary results that show possible involvement of noncoding RNAs and strategies using gene regulation and chemical libraries.
