Single and Ensemble Behaviors of Inorganic Nanoparticles
소속 :
연사 : Dr. Young-wook Jun (Department of Chemistry, University of California, Berkeley)
일시 : 2009-05-27 09:00 ~
장소 : 500동 L310호
일시: 2009년 5월 27일 오후 17:00
장소: 500동 L310호
-Abstract-
Single molecule imaging has enabled the exploration of molecular processes that are lost by extrapolation of ensemble assays. Although single molecule imaging techniques have advanced remarkably in the last decade, continuous observation of single molecule events with a high spatial resolution has remained challenging. In this talk, I will present new single molecule/particle imaging techniques to study behaviors of individual inorganic nanoparticles. At the beginning of this talk, I will discuss about growth kinetics of colloidal inorganic nanoparticles. While the classical nanoparticle growth model overlooked the particle-particle interaction, observation of single nanoparticle growth trajectories showed that strong inter-particle interactions are strongly involved for focusing size-distribution of nanoparticles. Also, I will present a single molecule imaging technique that uses plasmon coupling of metal nanoparticles to study apoptotic signaling processes in live cells. The “crown nanoparticle plasmon rulers” developed intensely scatter light without photobleaching and blinking. These properties allowed us to detect early caspase-3 activation and its cell-by-cell heterogeneity, which were not possible with conventional ensemble analyses.
장소: 500동 L310호
-Abstract-
Single molecule imaging has enabled the exploration of molecular processes that are lost by extrapolation of ensemble assays. Although single molecule imaging techniques have advanced remarkably in the last decade, continuous observation of single molecule events with a high spatial resolution has remained challenging. In this talk, I will present new single molecule/particle imaging techniques to study behaviors of individual inorganic nanoparticles. At the beginning of this talk, I will discuss about growth kinetics of colloidal inorganic nanoparticles. While the classical nanoparticle growth model overlooked the particle-particle interaction, observation of single nanoparticle growth trajectories showed that strong inter-particle interactions are strongly involved for focusing size-distribution of nanoparticles. Also, I will present a single molecule imaging technique that uses plasmon coupling of metal nanoparticles to study apoptotic signaling processes in live cells. The “crown nanoparticle plasmon rulers” developed intensely scatter light without photobleaching and blinking. These properties allowed us to detect early caspase-3 activation and its cell-by-cell heterogeneity, which were not possible with conventional ensemble analyses.
