MOLECULAR ENGINEERING OF MULTIFUNCTIONAL NUCLEIC ACID PROBES FOR BIOANALYTICAL AND BIOMEDICAL APPLICAT IONS
소속 :
연사 : 김영미 박사 (University of Florida, Department of Chemistry)
일시 : 2009-02-23 11:00 ~
장소 : 503동 L305호
일시: 2009년 2월 23일 오전 11:00
장소: 503동 L305호
- Abstract -
Over the past few decades, the role of nucleic acid including deoxyribonucleic acid and ribonucleic acids in purely biological systems has been defined, and these versatile compounds have also been integrated into nanoscience. As a result, many different types of DNA probes and architectures have been proposed to detect a wide range of targets, such as ions, small molecules, nucleic acids, proteins, and even living organisms. These developments have been accelerated by advanced technologies for modifying the nucleic acid-based probes with different signaling mechanism and bases of nucleic acids with organic functional groups. The overall goal of this doctoral research is dedicated to engineering these biomolecular components with the objective of building novel molecular tools and devices for biological studies, biomedical research and therapeutic applications. The first effort was focused on developing mRNA-tracking probes using nonstandard nucleic acids, such as L-DNAs, locked nucleic acids, 2’-OMe RNAs, to extend probe lifetimes and enhance the sensitivity and selectivity. The second line of research was concentrated on designing multifunctional nucleic acid inhibitors to enhance or create functions for pharmaceutical applications. The last one was development of an ultra sensitive heavy metal sensor. In summary, this research has focused on the design, synthesis and investigation of multifunctional and advanced nucleic acid probes, with the ultimate goal of increasing the understanding of biological processes and the development of advanced molecules for nucleic acid-based detection and therapy.
장소: 503동 L305호
- Abstract -
Over the past few decades, the role of nucleic acid including deoxyribonucleic acid and ribonucleic acids in purely biological systems has been defined, and these versatile compounds have also been integrated into nanoscience. As a result, many different types of DNA probes and architectures have been proposed to detect a wide range of targets, such as ions, small molecules, nucleic acids, proteins, and even living organisms. These developments have been accelerated by advanced technologies for modifying the nucleic acid-based probes with different signaling mechanism and bases of nucleic acids with organic functional groups. The overall goal of this doctoral research is dedicated to engineering these biomolecular components with the objective of building novel molecular tools and devices for biological studies, biomedical research and therapeutic applications. The first effort was focused on developing mRNA-tracking probes using nonstandard nucleic acids, such as L-DNAs, locked nucleic acids, 2’-OMe RNAs, to extend probe lifetimes and enhance the sensitivity and selectivity. The second line of research was concentrated on designing multifunctional nucleic acid inhibitors to enhance or create functions for pharmaceutical applications. The last one was development of an ultra sensitive heavy metal sensor. In summary, this research has focused on the design, synthesis and investigation of multifunctional and advanced nucleic acid probes, with the ultimate goal of increasing the understanding of biological processes and the development of advanced molecules for nucleic acid-based detection and therapy.
