Real-Time Measurements of Neurotransmission In Vivo Using Enzyme-Based Microelectrode Arrays
소속 :
연사 : Greg A. Gerhardt(University of Kentucky Medical Center)
일시 : 2011-11-14 11:30 ~
장소 : 500동 L305
일 시 : 2011년 11월 14일, 11:30 AM
장 소 : 500동 L305
-Abstract-
Perhaps the greatest problem in the use of microelectrodes for the detection of analytes in the brain involves the lack of specificity of the recording microelectrode for a given analyte. Historically, microelectrodes have not had the self-referencing capabilities of other techniques to allow for subtraction of noise and interferents. Our group has been working on a new generation of microelectrodes, which are micro-fabricated using photolithographic techniques. Our new designs employ Al2O3 substrates patterned with Pt or Ir surfaces and are coated with polyimide for insulation. The resulting 4-16 recording site arrays are modified with enzymes and organic molecules to make them sensitive and selective for the detection of a variety of neurochemicals, including but not limited to glutamate, glucose, aspartate, choline, acetylcholine, lactate, GABA and others. We have extensively focused on the development of microelectrodes for the reproducible, sensitive, selective and rapid measures of L-glutamate (Glu), which is the major excitatory neurotransmitter in the CNS.
장 소 : 500동 L305
-Abstract-
Perhaps the greatest problem in the use of microelectrodes for the detection of analytes in the brain involves the lack of specificity of the recording microelectrode for a given analyte. Historically, microelectrodes have not had the self-referencing capabilities of other techniques to allow for subtraction of noise and interferents. Our group has been working on a new generation of microelectrodes, which are micro-fabricated using photolithographic techniques. Our new designs employ Al2O3 substrates patterned with Pt or Ir surfaces and are coated with polyimide for insulation. The resulting 4-16 recording site arrays are modified with enzymes and organic molecules to make them sensitive and selective for the detection of a variety of neurochemicals, including but not limited to glutamate, glucose, aspartate, choline, acetylcholine, lactate, GABA and others. We have extensively focused on the development of microelectrodes for the reproducible, sensitive, selective and rapid measures of L-glutamate (Glu), which is the major excitatory neurotransmitter in the CNS.
