15795
이현우Associate Professor
  • E-MAILrheehw@snu.ac.kr
  • ADDRESS503-103B

  • OFFICE+82-2-880-4390FAX+82--LAB+82-2-880-2679

Current Research Interests
In a human cell, around 20,000 different human proteins are known to be expressed. These proteins are not uniformly distributed throughout the cell, but they are located in highly specific targeted spaces and form a variety of microenvironments. To map localized protein information in a sub-cellular "space of interest", we develop a new protein mapping method based on compatible protein label chemistry in living cellular physiology. This new method called proximity labeling has the potential to uncover new protein components that are not mapped by traditional protein purification methods such as co-immunoprecipitation. This method can be extended to uncover spatiotemporal subcellular distribution of other biomolecules such as nucleic acids and metabolites in living cells and in live animals.

Education
B.S. Biological Sciences and Chemistry (Double Major), Seoul National University, Korea, 2004
Ph.D. Organic Chemistry, Seoul National University, Korea, 2009

Research Experiences
Postdoctoral Researcher, MIT Chemistry, USA, 2010-2012
Assistant Professor, UNIST Chemistry, Korea, 2013-2017
Associate Professor, UNIST Chemistry, Korea, 2017-2018

Representative Publications
Lee SY, Kang MG, Shin S, Kwak C, Kwon T, Seo JK*, Kim JS*, Rhee HW*, Architecture Mapping of the Inner Mitochondrial Membrane Proteome by Cheimcal Tools in Live Cells. J. Am. Chem. Soc. 2017, 139, 3651-3662.

Lee SY†, Lee H†, Lee HK, Lee SW, Ha SC, Kwon T, Seo JK*, Lee C*, Rhee HW* Proximity-Directed Labeling Reveals a New Rapamycin-Induced Heterodimer of FKBP25 and FRB in Live Cells. ACS Cent. Sci. 2016, 2, 506-516. (†equally contributed)

Lee SY, Kang MG, Park JS, Lee G, Ting AY*, Rhee HW* APEX fingerprinting reveals the subcellular localization of proteins of interest. Cell Rep. 2016, 15, 1837-1847

Rhee HW†, Zou P†, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY* Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging. Science 2013, 339, 1328-1331. (†equally contributed)

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