15637
이남기Associate Professor
  • E-MAILnamkilee@snu.ac.kr
  • ADDRESS503-403,132

  • OFFICE+82-2-880-2234FAX+82--LAB+82-2-880-2235

Current Research Interests
The main research interest of our group is developing new single-molecule and single-cell imaging techniques for investigating various biological phenomena. We are using single-molecule FRET, single-vesicle assay, single-protein detection in a living cell, real-time single-protein tracking, and super-resolution imaging. Using these techniques, we are directly visualizing the cellular processes, such as transcriptional dynamics and the movement of DNA, in a living cell. We are also heavily investigating membrane protein interactions and their diffusional mode in a living cell. Not only the living cell system, we are also studying protein-DNA interactions at the single-molecule level. Finally, we are studying the molecular mechanism of Parkinson’s disease based on the interactions between alpha-Synuclein and SNARE-proteins.

Education
Ph.D. Chemistry, Seoul National University, Seoul, Korea, 2005
M.S. Chemistry, Seoul National University, Seoul, Korea, 2000
B.S. Chemistry, Seoul National University, Seoul, Korea, 1998

Research Experiences
Associate Professor, Department of Chemistry, Seoul National University, Seoul, Korea, 2017-Present

Associate Professor, Department of Physics & School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea, 2013-2017

Assistant Professor, Department of Physics & School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Korea, 2009-2013

Postdoctoral Fellow, Harvard University, Cambridge, USA, 2006-2008

Visiting Scientist, U. C. Los Angeles, California, USA, 2002-2003

Representative Publications
“Dynamic release of bending stress in short dsDNA by a kink and forks”, C. Kim, O. Lee, J.-Y. Kim, W. Sung, N. K. Lee,* Angew. Chem. Int. Ed. 54, 8943–8947 (2015).

“Real-time sub-millisecond single-molecule FRET dynamics of freely diffusing molecules with liposome tethering”, J.-Y. Kim, C. Kim, N. K. Lee,* Nature Communications 6, 6992 (2015).

“Analysis of Interactions between the Epidermal Growth Factor Receptor and Soluble Ligands on the Basis of Single-Molecule Diffusivity in the Membrane of Living Cells”, D.-H. Kim, K. Zhou, D.-K. Kim, S. Park, J. Noh, Y. Kwon, D. Kim, N. W. Song, J.-B. Lee, P.-G. Suh, N. K. Lee*, S. H. Ryu,* Angew. Chem. Int. Ed. 54, 7028–7032 (2015).

“Contribution of RNA polymerase concentration variation to protein expression noise”, S. Yang, S. Kim, Y. R. Lim, C. Kim, H. J. An, J-H. Kim, J. Sung*, N. K. Lee,* Nature Communications 5, 4761 (2014).

“Large α-Synuclein oligomers inhibit neuronal SNARE-mediated vesicle docking”, B. K. Choi, M.-G. Choi, J.-Y. Kim, Y. Yang, Y. Lai, D.-H. Kweon, N. K. Lee,* Y.-K. Shin,* Proc. Natl. Acad. Sci. USA 110, 4087–4092 (2013).

“Solution single-vesicle assay reveals PIP2 is required for the sequential actions of synaptotagmin-1 on SNAREs”, J.Y. Kim, B. K Choi, M. G Choi, S. A. Kim, Y. K. Shin,* N. K Lee,* EMBO J. 31, 2144-2155 (2012).

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